will be suggested. Essential requirements in culture media Any culture medium must contains: -A source of energy -Sources of carbon, nitrogen, sulfur, phosphorus -Minerals, e.g., Ca2+, Mg2+, Na+ -Vitamins and growth factors - Water 12/30/13 Dr. Shyamal Kr Paul, Culture media 2 Clipping is a handy way to collect important slides you want to go back to later. You can change your ad preferences anytime. They have added ingredients.They have added ingredients.